ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
전자를 '고정상', 후자를 '이동상'이라 부르며 크로마토그래피에서는 분석자는 고정상과 이동상의 조합에 의해 분석물의 분리를 제어할 수 있게 됩니다.따라서 분석물, 고정상, 이동상, 세 가지 특성의 이해가 크로마트그래피에서 매우 중요합니다.
, which will allow us to explore a broad choice of cell phases with only seven experiments. We get started by modifying the quantity of acetonitrile within the cell stage to create the very best separation inside the specified analysis time.
are developed by reacting the silica particles with an organochlorosilane of the final kind Si(CH3)2RCl, where R is an alkyl or substituted alkyl group.
. The working pump plus the equilibrating pump each Use a piston whose back and forth motion maintains a constant movement rate of nearly a number of mL/min and gives the high output stress needed to press the cellular period throughout the chromatographic column.
The interface amongst the HPLC and the mass spectrometer is technically more difficult than that inside of a GC–MS due to the incompatibility of the liquid cellular period With all the mass spectrometer’s high vacuum need.
. Block diagram of an HPLC–MS. A three component combination enters the HPLC. When ingredient A elutes in the column, click here it enters the MS ion supply and ionizes to sort the mother or father ion and a number of other fragment ions.
Quite a few differing types of detectors happen to be use to monitor HPLC separations, a lot of which make use of the spectroscopic tactics from Chapter ten or maybe the electrochemical methods from Chapter 11.
Retention instances: Time it takes for every analyte to get to the detector, furnishing a attribute fingerprint for identification.
The focus of polynuclear aromatic hydrocarbons (PAH) in soil is determined by initial extracting the PAHs with methylene chloride. The extract is diluted, if required, plus the PAHs divided by HPLC using a UV/Vis or fluorescence detector. Calibration is attained employing a number of external criteria. In a standard Examination a 2.013-g sample of dried soil is check here extracted with twenty.
溶媒の組成に勾配を付けて（すなわち組成を連続的に変えて）溶出を行うことも多い。たとえば後述の逆相クロマトグラフィーにおいて水/メタノール勾配を使う場合、まずメタノールの少ない条件で極性の高い物質が溶出し、その後メタノールの割合を増加させてゆくに従ってより極性の低い物質が順次溶出する。これをグラジェント分析と呼ぶ。これに対し、一定組成の溶媒で分析物を溶出させる分析法をアイソクラテック分析と呼ぶ。
HPLC is actually a enhanced kind of column chromatography. The main difference is, here instead of dripping solvent under gravity a pressure of as many as 400 environment is utilized on the chromatography to have a quick separation.
An HPLC normally involves two columns: an analytical column, which can be liable for the separation, and also a guard column which is placed prior to the analytical column to shield it from contamination.